Irvine Chemistry Lab
X-Lyz™ Protein Assay Kit

Total Protein Quantitation
 



Features

  • No need to generate standard curve each
    time, saving several hours of workload
  • Wide linear range up to 10,000µg/mL
  • Quick and simple procedure
  • Low protein variations
  • Simple procedure to remove interferents
  • Excellent shelf life stability
 



Contents

  1. X-Lyz™ Protein Assay Reagent A
  2. X-Lyz™ Protein Assay Reagent B
  3. TCA Precipitation Solution (for interferent
    removal)
  4. BSA Standard
  5. User Instructions
 
Please see the MSDS for safely handling.
 
Contact us for testing services and new applications..
 



Product Description

The  X-Lyz™ protein assay is a simple
procedure to measure the total protein
concentration in solution and solid.
The proprietary assay involves a
reaction with primary amines of
proteins to form a chromophore. It
exhibits strong UV absorbance at 350-
405nm linearly proportional to
concentrations over a broad range of
10-10,000µg/mL.  The rapid color
development is complete after 15min
incubation.   A study shows the
calibration is stable over a year; it
does not require to re-generate the
standard curve each time.

The X-Lyz™ assay kit can also be
used in other amine detection
applications, such as lysine
determination, proteolysis process
monitoring, cross-linking density
estimation, and polyamine
measurement.
 
 
 
Catalog #
User
Instructions
# of Assays
Microplate
# of Assays
Test Tube
Price
 
IC286094-1
Download PDF
3200
480
$48
IC286094-2
Download PDF
6400
960
$68
IC286094-4
Download PDF
12800
1920
$120

Introductory Video
 
 
 



Broad Linear Range

The X-Lyz™ assay has a broad linear range up to
10,000µg/mL (
Figure 1), while other methods, such as
BCA and Bradford assays, are lower than 2000µg/mL.

Figure 1: Standard Curve of BSA. The analysis was conducted
with sample size 25 µL and WR/Sample 10/1 in 96 well
microplate.
 


Accurate Estimation

The estimation of the protein samples has an excellent
agreement with the true values as shown in
Table 1.



Table 1: Estimates of BSA Samples from the Standard Curve in
Figure 1.
 
True Values
(µg/mL)
Average
Absorbance
Estimates
(µg/mL)
Deviations
200
0.049
215.1
7.5%
300
0.074
301.2
0.4%
600
0.160
597.4
-0.4%
900
0.260
941.9
4.7%
1900
0.562
1982.1
4.3%
5000
1.406
4889.2
-2.2%
9000
2.600
9001.8
0.0%
 


Long Calibration Stability

The kit has shown a highly repeatable performance
and does not require to generate standard curve each
time.  In a stability study, twelve different assays were
conducted over a year using the same standard curve.
The average absolute deviations of the estimates were
shown to be smaller than 4%, while the maximum was
within 10% (
Table 2).

Table 2: Deviations of Estimates of Protein Samples. The
analysis was conducted with sample size 75 µL and
WR/Sample 1/1 in 96 well microplate.
True Values: The true concentrations of BSA samples in µg/mL.
Average Absorbance: Average absorbance in five replicates.
Estimates: Estimates of sample concentrations.
Deviations: Percentage deviations of the estimates over the true values.
 



Low Level of Protein Variation

The X-Lyz™ reagent has shown a less variation among
different proteins compared to other assays (
Table 3).




Table 3: Comparison of Protein Variations in Different Methods
 
Concentration
Average
Minimum
Maximum
100µg/mL
3.8%
0.9%
7.0%
200µg/mL
3.1%
0.2%
9.0%
300µg/mL
2.7%
0.3%
6.9%
600µg/mL
2.9%
0.7%
6.1%
900µg/mL
1.7%
0.3%
3.3%
1900µg/mL
2.7%
0.6%
5.4%
Assays
X-Lyz™
BCA
Pierce 660
Bradford
Coefficient of
Variation
8.7%
15%
37%
44%
 
Concentration: The true concentrations of the tested proteins samples.
Average: The average deviations of the estimates in twelve assays.
Minimum: The minimum deviations of the estimates in twelve assays.
Maximum: The maximum deviations of the estimates in twelve assays.